Anti-Phosphorylated Neurofilament NF-H NEFH Antibody

Product Name

Anti-Phosphorylated Neurofilament NF-H NEFH Antibody

View all NF-H Antibodies

SKU/Catalog Number

M05307-7

Size

50ul

Description

Boster Bio Anti-Phosphorylated Neurofilament NF-H NEFH Antibody catalog # M05307-7. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Canine, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phosphorylated Neurofilament NF-H NEFH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05307-7)

Host

Chicken

Contents

Supplied as an aliquot of IgY preparation plus 5mM NaN₃

Clonality

Polyclonal

Immunogen

Native NF-H purified from bovine spinal cord.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M05307-7 is reactive to NEFH in Bovine, Canine, Equine, Human, Mouse, Pig, Rat

Applications

M05307-7 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

112.479kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000,000. IF/ICC and IHC: 1:100,000. ELISA-capture antibody

Validation Images & Assay Conditions

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Western blot analysis of spinal cord lysates from different species using chicken pAb to NF-H, M05307-7, dilution 1:20,000 in green: [1] protein standard (red), [2] rat, [3] mouse, and [4] cow spinal cord. Strong band at about 200-220kDa corresponds to the phosphorylated from of NF-H. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H (not shown, but see reference 1).

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Immunohistological analysis of a rat cerebellum section stained with chicken pAb to NF-H, M05307-7, dilution 1:5,000 in red, and costained with rabbit pAb to GFAP, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free floating sections were stained with above antibodies. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

Gene/Protein Information For NEFH (Source: Uniprot.org, NCBI)

Alternative Names

200 kDa neurofilament protein; KIAA0845; NEFH; Neurofilament Heavy (200kDa); neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide; NF200; NFH; NF-H NEFH CMT2CC, NFH neurofilament heavy neurofilament heavy polypeptide|200 kDa neurofilament protein|NF-H|neurofilament triplet H protein|neurofilament, heavy polypeptide 200kDa

For more info on NEFH, check out the NEFH Infographic

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In this infographic, you will see the following information for NEFH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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